Mouse Serum

Mouse Serum

Sterile filtered mouse serum

Alias:

Normal Mouse Serum, mouse serum, normal mouse serum

Product Description: Premium Mouse Serum (special promotion)

Product specification: 30ml 100ml 500ml

Grade: High purity ≥99.9%

KM mouse serum was sterilized by 0.1um sterile filtration

● Provide basic nutrients: amino acids, vitamins, inorganic substances, lipid substances, nucleic acid derivatives, etc., is a necessary substance for cell growth.

● Provide hormones and various growth factors: insulin, adrenocortical hormones (hydrocortisone, dexamethasone), steroid hormones (estradiol, testosterone, progesterone), etc. Growth factors such as fibroblast growth factor, epidermal growth factor, platelet growth factor, etc.

● Provide binding protein: Binding protein function is to carry important low molecular weight substances, such as albumin carries vitamins, fats, and hormones, and transferrin carries iron. Binding proteins play an important role in cell metabolism.

● Provide contact promoting and stretching factors to prevent cell adhesion from mechanical damage.

● Some protective effects on cells in culture: some cells, such as endothelial cells and myeloid cells, can release protease, and serum contains anti-protease components to play a neutralizing role. This effect was discovered by chance, and serum was used to terminate trypsin digestion. Trypsin has been widely used in the digestion and passage of adherent cells. Serum proteins form the viscosity of the serum, which protects cells from mechanical damage, especially when stirred in suspension culture. Serum also contains some trace elements and ions, they play an important role in metabolic detoxification, such as SeO3, selenium and so on Avoid creating sediment.

1. When thawing the serum, please follow the recommended gradual thawing method (-2℃ to 4℃ to room temperature). If the temperature of the serum changes too much during thawing (such as -20℃ to 37℃), the experiment shows that it is very easy to produce precipitate.

2, when thawing the serum, please shake it evenly at any time, so that the temperature and composition are uniform, reduce the occurrence of precipitation

3. Do not place the serum at 37℃ for too long. If placed at 37℃ for too long, the serum will become cloudy, and many of the more unstable components of the serum will also be damaged, and affect the quality of the serum.

4, the heat inactivation of serum is very easy to cause the increase of sediment, if not necessary, you do not need to do this step.

5. If you must do heat inactivation of serum, please abide by the principle of 56℃, 30 minutes, and shake evenly at any time. The temperature is too high, the time is too long or the shaking is not uniform, will cause the increase of sediment. How to deal with the occurrence of flocculent precipitates found in serum after thawing? To remove these flocculent precipitates, the serum can be divided into a sterile centrifuge tube, slightly centrifuged at 400g, and the supernatant can then be added to the medium for filtration. However, it is not recommended to remove these flocculent sediments by filtration because it may block the filtration membrane.

Application: The mouse serum can be used in most immunological experiments, as a blocking solution or blank control, etc.

Whole blood:

(1) Preparation of serum: The obtained blood can not be added with anticoagulant, and is placed in a centrifuge tube or a vessel that can be centrifuged on an inclined plane at an Angle of 20-30°, standing or placed in an environment of 37 ° C to promote coagulation. After coagulation, the blood is centrifuged (generally 3000rpm-6000rpm for 5 ~ 10min), and the supernatant obtained is the serum. The supernatant can be carefully sucked out (do not suck out the cell components) and divided into spare.

(2) Plasma preparation: Add a certain proportion of anticoagulant (anticoagulant: blood = 1:9, add the blood to a certain amount, mix it upside-down, and the supernatant obtained after centrifugation (centrifugation conditions are the same as above) is plasma. It is better for the initial user to move the supernatant to another cleaning container, and gradually suck the plasma down with a capillary straw attached to the liquid level, and do not suck up the cell components.

(3) Preparation of platelet-rich plasma: The obtained blood was centrifuged at 800rpm for 5min, and its supernatant was platelet-rich plasma.

Note: It is best to let the blood drop when collecting blood, and do not touch the wall of the test tube. Hemolysis is likely to be caused by unclean collection vessels sticking to hair. When collecting, try to avoid debris such as animal hair and water hanging on the tube wall. Shake the collection tube violently, and high centrifugal speed is easy to cause hemolysis.

Winter prone to jelly shape, solution

1. The removed blood is placed at room temperature (not on ice) or in a 37 degree water bath for one hour, and the serum can be centrifuged after precipitation

2. Centrifugal speed 3000-6000rpm, 10min, room temperature

3. If there is still jelly, it is recommended to stir the blood clockwise with the pipette head and centrifuge it again at 6000rpm for 5min. The effect is very good.

Storage and security

Storage: -20℃ storage