Horse serum
Time: 2023-03-16 Edited by: Thomas
Horse serum is collected from the blood of healthy horses as raw material, which is collected aseptically in a closed system and filtered by micropores. The characteristics of horse serum are clear liquid, no phage, ultra-low endotoxin, no hemolysis, no foreign body, no bacterial fungus mycoplasma plasmoplasma and chlamydia, etc., which has excellent effect on promoting cell growth and reproduction, and is suitable for cell, tissue and organ culture, cell line preservation, and monoclonal antibody development. It is one of the important medium for hospitals, scientific research institutions, animal vaccines and biopharmaceutical manufacturers. Cells cannot survive in pure culture medium, and certain nutrients and growth factors must be provided in special types of cell culture for cells to grow and maintain their growth state.
Serum is often added to basic medium, and the final serum concentration is usually 5-20%. The source of serum for special purposes must be determined empirically. The serum types widely used are horse serum and bovine serum, fetal bovine serum rich in mitotic factors, often selected as the serum for cell proliferation, but also used in cell lines and primary culture. Horse serum is often used in post-mitotic neuron culture.
However, many people also use fetal bovine serum for neuron culture and horse serum for glial cells. Horse serum is a natural product, rich in protein, which can replace FBS for the culture of most mammalian cells and should not be used for the culture of insect cells. Horse serum contains more growth factors, especially for hematopoietic stem cell culture. In addition, horse serum can also be used in the culture of primary nerve cells and mycoplasma.
Horse serum is collected from the blood of healthy horses as raw material, which is collected aseptically in a closed system and filtered by micropores. The characteristics of horse serum are clear liquid, no phage, ultra-low endotoxin, no hemolysis, no foreign body, no bacterial fungus mycoplasma plasmoplasma and chlamydia, etc., which has excellent effect on promoting cell growth and reproduction, and is suitable for cell, tissue and organ culture, cell line preservation, and monoclonal antibody development. It is one of the important medium for hospitals, scientific research institutions, animal vaccines and biopharmaceutical manufacturers. Cells cannot survive in pure culture medium, and certain nutrients and growth factors must be provided in special types of cell culture for cells to grow and maintain their growth state.
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