characteristic |
Polyclonal antibody |
Monoclonal antibody |
The immunogen requirement |
High immunogen purity means high antibody purity |
High purity antibodies can be obtained from impure immunogens |
Antibody-producing cell |
polyclonal |
monoclonal |
homogeneity |
Highly heterogeneous |
Highly homogenous |
specificity |
Higher, binding to a variety of epitope on the antigen |
High, bound to a specific determinate |
stability |
better |
Relatively poor, sensitive to physical and chemical conditions |
standardization |
Difficult, different batches of antibody quality varies greatly |
Easy to standardize, small differences between batches |
Cross reaction |
It's very common. It's hard to avoid nonspecific reactions |
Infrequent, non-specific reactions can be avoided |
Precipitation reaction |
There are |
Most don't |
Applicable serological tests |
Most serological tests |
One or more, to be selected appropriately |
supply |
limited |
infinite |
Effective antibody content |
0.1~1.0 |
General suspension culture 0.01~0.05, hollow |
Fiber and other reactor culture 1.0~5.0, small |
||
Rat abdominal water 1.0~5.0 |
||
Regardless of Ig content |
10.0~15.0 |
"There is generally no mouse ascites in vitro |
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